The vertebrate kidney has an innate ability to regenerate after acute kidney injury (AKI) that is highly conserved across species. During regeneration, the same transcription factors normally expressed during organogenesis are reactivated. With Dr. Neil Hukriede, Department of Developmental Biology, we are using high throughput screening in zebrafish embryos to discover molecules that activate organogenesis, and have found agents that ameliorate recovery from AKI and reduce fibrosis in mouse models when administered days after the injury has occurred.
Sanker et al. Development of high-content assays for kidney progenitor cell expansion in transgenic zebrafish. Skrypnyk, N.I., et al., Am J Physiol Renal Physiol, 2016. Delayed treatment with PTBA analogs reduces post injury renal fibrosis after kidney injury. Am J Physiol Renal Physiol. 2015:ajprenal 00503 2015. Hukriede et al. Drug Discovery to Halt the Progression of Acute Kidney Injury to Chronic Kidney Disease: A Case for Phenotypic Drug Discovery in Acute Kidney Injury. Nephron. 2017. doi: 10.1159/000476079. PubMed PMID: 28614822.